Pulse oximetry is a well-established technique in nowadays human and veterinarian medicine. Also in the farm animal sector, it could be a useful tool to detect critical conditions of the oxygen supply and the cardiovascular system of the patient. However, its use in ruminant medicine is still limited to experimental application. The objective of this study was to evaluate the accuracy of a Radius-7 Wearable Pulse Oximeter (Masimo Corporation, Irvine, CA) for monitoring the vital parameters of Holstein Frisian calves. The sensor of the pulse oximeter was placed in the interdigital space of the calf’s front leg. The arterial oxygen saturation (SO2) of 40 newborn calves was measured and compared with the corresponding results from a portable blood gas analyzer which served as reference method. The arterial blood sample was taken from the medial intermediate branch of the caudal auricular artery. The pulse rate was measured on 10 calves aged 0 to 7 days with the pulse oximeter and a heart rate belt simultaneously and their level of agreement was evaluated. Spearman correlation coefficient was 93.8 % for the SO2 parameter between the pulse oximeter and the blood gas analyzer and 97.7 % for the pulse rate between the pulse oximeter and the heart rate belt. The pulse oximeter overestimated the SO2 by 2.95 ± 6.39 % and underestimated the pulse rate by -0.41 ± 3.18 bpm compared with the corresponding reference methods. This pulse oximeter seems to be suitable for continuous monitoring of SO2 and pulse of Holstein-Friesian calves.