A Field experiment was conducted to estimate crop nitrogen (N) status and chlorophyll content in wheat crop by using chlorophyll content meter(Apogee’s CCM-200) and N-Tester®  (Make YARA International). The experiment was conducted by sowing university recommended wheat variety viz. PBW 550 with 5 nitrogen levels i.e. 0, 30, 60, 90, 120 & 150 kg N/ha. It was found that at tillering stage when nitrogen rates were increased from 0 to 150 kg ha^-1 , the relation with both the CCI meter and N-Tester (NT) for determination of plant nitrogen were better correlated having Pearson’s coefficient 0.939 and 0.926 respectively. However, at booting and ear emergence both CCI and NT does not showed good correlations for the determination of N-content in plant leaves. But at crop growth stages like tillering, booting and ear emergence, Lab N content showed a strong curvilinear relationship with CCI having coefficient of determination 0.975, 0.883 and 0.873 respectively. Coefficient of determination values for N tester and lab N-content also showed good polynomial relationship having values 0.944, 0.928 and 0.827 at tillering, booting and ear emergence stages respectively. For chlorophyll content measurement, both of the instruments chlorophyll meter and N-tester have shown good correlations having Pearson’s coefficient ranging from 0.698 to 0.930 at all  growth stages of crop.  The chlorophyll content measured in laboratory also showed a strong curvilinear relationship with CCI at tillering, booting and ear emergence stage with coefficient of determination 0.952, 0.966 and 0.968, respectively. Coefficient of determination values between N tester and chlorophyll content showed polynomial relationship having values 0.932, 0.983 and 0.983 for tillering, booting and ear emergence stages respectively.  It was also concluded that there was a strong polynomial relationship between chlorophyll content and lab N  in plant leaves with coefficients of determination 0.898, 0.916 and 0.937 at tillering, booting and ear emergence stages respectively. The grain yield obtained with N application of 120 Kg ha^-1 was 91.1, 38.4 and 10.2 per cent higher over the application of 30, 60 and 120 Kg N ha^-1, respectively.