Protected horticulture in greenhouses and plant factories has been increased in many countries due to the advantages of year-round production in controlled environment for improved productivity and quality. For protected horticulture, environmental conditions are monitored and controlled through wired and wireless devices. Various devices are used for monitoring and control of spatial and temporal variability in crop growth environmental conditions. Recently, various sensors and control devices, and also wireless communication tools have been adopted for efficient monitoring and control of the greenhouse environments. Sensing parameters may include light intensity, temperature, humidity, CO₂, wind, and rain for ambient environment, and EC, pH, and nutrient contents for root zone environments. Control devices may include lamp, heater, cooler, humidifier, dehumidifier, fan, CO₂ generator, and window motor for ambient environment, and nutrient and water supply devices for root zone environment. One of the major problems in those sensing and control devices is low compatibility among the units, due to company and/or user customized specifications of type (e.g., voltage, current, and pulse), range (e.g., 0~20 mA, 0~5 V, and 0~12 V), and communication protocol (e.g., analogy, digital, and RS-232) of input and output signal. To solve these problems and improve the compatibility, a sensor and interface module was fabricated. In this paper, the developed sensor and control interface module was evaluated for commercially available various sensors and control devices. First, commercially available sensors and control devices were surveyed, and several units were selected considering the type, range, and communication protocol of input and output signal. Then performance of the developed sensing and control interface module was evaluated in farmers’ fields and a test bed. The sensing and interface module would be improved through practical feasibility tests in different greenhouses.